Bicine
瀹冩槸涓�绋叐鎬ч洟瀛愮珐娌栨恫锛屽畠鑸囪ū澶氬父瑕嬮噾灞舰鎴愬挤(qi谩ng)绲�(lu貌)鍚堢墿锛涘洜姝わ紝绌�(w臎n)瀹氬父鏁�(sh霉)閬告搰姝ょ珐娌栨恫鏅�(sh铆)鎳�(y墨ng)鑰冩叜婵冨害銆傚畠鍙敤浜庤泲鐧借唱(zh矛)绲�(ji茅)鏅剁殑瑙ｆ焙鏂规鍜屼綔鐐虹Щ鍕�(d貌ng)闄介洟瀛愪氦鎻涜壊璀滀腑鐨勭浉绶╂矕娑插拰娲楄劔娑�銆�
MES
鐢ㄤ簬鍚噾灞洟瀛愮殑婧舵恫涓紝鍥�?y脿n)妯楊幗绮�?hu矛)鑸囧ぇ澶氭暩(sh霉)閲戝爆褰㈡垚绲�(lu貌)鍚堢墿銆侻ES閫氬父鐢ㄤ簬閰垫瘝锛岀窗(x矛)鑿屽拰鍝轰钩鍕�(d貌ng)鐗╃窗(x矛)鑳炵殑绶╂矕鍩归(y菐ng)鍩轰腑锛屼絾婵冨害楂樹簬10 mM鏅�(sh铆)灏�(du矛)妞嶇墿鏈夋瘨銆侻ES鍦ㄥ悇绋壊璀滃拰闆绘吵瀵�(sh铆)椹�(y脿n)涓篃鏄緢濂界殑绶╂矕娑查伕鎿�锛屽洜?y脿n)妯楊徍@绀哄嚭杓冧綆鐨勯洟瀛愰伔绉荤巼鍜岄浕灏�(d菐o)鐜�銆傚湪pH鍊肩偤5.5鑷�6.7鐨勮寖鍦嶅収(n猫i)锛屽畠鏄竴绋湁鏁堢殑绶╂矕鍔戙��
MOPS
甯哥敤浜庣窗(x矛)鑿�銆侀叺姣嶅拰鍝轰钩鍕�(d貌ng)鐗╃窗(x矛)鑳炵殑鍩归(y菐ng)鍩恒�傚畠閫氬父鐢ㄤ簬绱�(x矛)鑳炲煿椁�(y菐ng)鍩�锛屼綔鐐洪浕娉充腑鐨勯亱(y霉n)琛岀珐娌栨恫鍜岃泲鐧借唱(zh矛)绱斿寲銆侻OPS琚獚(r猫n)鐐烘槸鐢ㄤ簬鍦ㄧ搳鑴傜硸涓垎闆NA鐨勬サ浣崇珐娌栨恫鍑濊啝锛岀敱浜庣櫦(f膩)鐢熺殑楂樺垎杈ㄧ巼鍒嗛洟鍜岀己涔忕浉浜掍綔鐢∕OPS鍜孯Nase鎶戝埗鍔慏EPC涔嬮枔鐨勫樊鐣�銆傚畠涔熻瑕栫偤鐢ㄩ�擲DS-PAGE涓殑閬�(y霉n)琛岀珐娌栨恫銆�
MOPSO
瀹冪己涔忚垏澶у鏁�(sh霉)閲戝爆闆㈠瓙褰㈡垚绲�(lu貌)鍚堢墿鐨勮兘鍔�锛屼竴鑸敤浣滈噾灞洟瀛愭憾娑蹭腑鐨勯潪閰嶄綅绶╂矕娑层�傚畠鐢ㄤ綔姣涚窗(x矛)绠￠浕娉充腑鐨勮級楂旈浕瑙ｈ唱(zh矛)锛屼互鍙婅胺鑳辩敇鑲藉悎鎴愰叾鐨勭祼(ji茅)鏅剁珐娌栧姂锛涘湪鐔掑厜鍏夎瓬娉�锛屽垎鍏夊厜搴︽硶鍜岀瓑婧淮瀹氶噺鐔辨硶涓娇鐢�锛涜垏鐗涜娓呯櫧铔嬬櫧锛圔SA锛夌殑鑲介鏋剁浉浜掍綔鐢�锛岄槻姝SA鐔辫畩鎬�锛涚敤浣滅珐娌栫偔閰垫瘝鎻愬彇鐗╁煿椁�(y菐ng)鍩虹殑绲勬垚鎴愬垎锛涚敤浣滄脯(c猫)瀹氱敓鐗╂不鐞嗘捣娲嬪師娌瑰壇鐢�(ch菐n)鐗╃殑绶╂矕楂旂郴銆�
PIPES
瀹冨(sh铆)闅涗笂涓嶆憾浜庢按锛屼絾濡傛灉娓搁洟閰稿鍔燩IPES鐨勬憾瑙ｅ害鐢∟aOH灏囧叾杞�(zhu菐n)鍖栫偤閳夐菇銆傜己涔忚垏澶у鏁�(sh霉)閲戝爆闆㈠瓙褰㈡垚閲嶈绲�(lu貌)鍚堢墿鐨勮兘鍔涳紝涓�鑸敤浣滈噾灞洟瀛愭憾娑蹭腑鐨勯潪閰嶄綅绶╂矕娑�銆傝┎绶╂矕鍗�(q奴)鑳藉褰㈡垚鑷敱鍩猴紝鍥犳涓嶉仼鐢ㄤ簬姘у寲閭勫師鍙嶆噳(y墨ng)銆傞洊鐒禤IPES鍙敤浣滈櫧闆㈠瓙浜ゆ彌鑹茶瓬涓殑绶╂矕娑�锛屼絾瀹冩噳(y墨ng)瑭茬敱浜庣浉灏�(du矛)杓冨ぇ鐨勯洟瀛愬挤(qi谩ng)搴﹀拰婵冨害灏�(du矛)pKa鐨勪緷璩存��銆侾IPES琚獚(r猫n)鐐烘槸涓�绋サ濂界殑鍥哄畾绶╂矕娑茬敤浜庨浕瀛愰’寰彙鐨勬鐗╃窗(x矛)鑳�銆�
HEPES
瀹冭兘杓冮暦(zh菐ng)鏅�(sh铆)闁撴帶鍒舵亽瀹氱殑pH鑼冨湇锛屼綆婵冨害鐨凥EPES灏�(du矛)绱�(x矛)鑳炵劇姣掓�т綔鐢�锛屽父鐢ㄤ簬绱�(x矛)鑳炲煿椁�(y菐ng)锛屾瘮濡傚悇绋窗(x矛)鑿�銆佺湡鑿岀殑鍩归(y菐ng)娑叉垨鐥呮瘨淇濆瓨娑蹭腑鍙互閫氶亷娣诲姞HEPES渚嗙董鎸佺窗(x矛)鑳炪�佺窗(x矛)鑿�銆佺梾姣掓墍闇�鐨刾H鐠�(hu谩n)澧冦�傚彟澶�锛屽畠鑳藉共鎿綝NA鍜岄檺鍒舵�у収(n猫i)鍒囬叾涔嬮枔鐨勫弽鎳�(y墨ng)锛屼絾绋嬪害浣庝簬椤炰技鐨勫叿鏈夎純灏戝彇浠ｈ兒鍩虹殑绶╂矕娑�锛岄�欑ó缂轰箯骞叉摼鐨勭櫦(f膩)鐢熶富瑕佹槸鍥�?y脿n)姗榺(zh矛)瀛愬寲鍙旇兒鐨勭┖闁撲綅闃诲湪HEPES涓厑瑷辫垏DNA鐨勮矤(f霉)闆昏嵎姣斿ぇ澶氭暩(sh霉)鏇村急銆�
HEPPSO
HEPPSO宸茶鐢ㄤ綔绯栧師鍚堟垚閰剁殑绲�(ji茅)鏅剁珐娌栨恫閰跺拰鑸嘓EPPSO寰�(f霉)鍚堢殑閰�銆侶EPPSO灏�(du矛)鎸囩ず鐗╃óDaphniamagna骞句箮娌掓湁姣掓��锛屽洜姝よ瑾�(r猫n)鐐洪仼鍚堟瘨鐞嗗(xu茅)鐮旂┒銆傝┎绶╂矕鍗�(q奴)鑳藉褰㈡垚鑷敱鍩猴紝鍥犳涓嶉仼鐢ㄤ簬姘у寲閭勫師鍙嶆噳(y墨ng)銆侶EPPSO褰㈡垚涓�鍊�(g猫)鑸嘋u2+闆㈠瓙绲�(lu貌)鍚堬紱鍥犳锛岀┅(w臎n)瀹氭�у父鏁�(sh霉)鍜屾績搴︽噳(y墨ng)鐐哄湪鍚湁閵呴洟瀛愮殑婧舵恫涓娇鐢ㄨ┎绶╂矕娑叉檪(sh铆)瑕佽�冩叜鍒伴�欎竴榛�(di菐n)銆傞洊鐒禜EPPSO鑸囬妳闆㈠瓙绲�(ji茅)鍚堬紝浣嗗畠浠嶇劧鏄仼鐢ㄤ簬浜岃緵鍙閰�銆�
TAPS
浣滅偤DNA绡╁垎楂旂郴涓父鐢ㄧ殑绶╂矕娑查珨绯�锛屼篃鍙綔鐐篟NA妯ｅ搧鐨勭珐娌栨恫鎴愬垎锛涢仼鐢ㄤ簬钁夌稜楂旇杽灞ゅ倷妯ｇ殑闆诲瓙鍌抽仦鍜岀７閰稿寲鐮旂┒锛涘彲浣滅偤姣涚窗(x矛)绠″崁(q奴)甯堕浕娉抽��(j矛n)琛岃泲鐧藉井閲忓垎鏋愮殑鑳屾櫙闆昏В璩�(zh矛)锛涜兘缍寔鍙嶆噳(y墨ng)楂旂郴鐨刾H鍊笺�傚父鐢ㄤ簬涓�浜涚敓鍖栬ê鏂疯│鍔戠洅銆丳CR瑷烘柗瑭﹀姂鐩掍互鍙奃NA/RNA鎻愬彇绛夎│鍔戠洅鐣�(d膩ng)涓�銆�